Spore strip for Hydrogen Peroxide (10^5 spores)

BT92/5 / Spore Strips Biological Indicator for Plasma or Vaporized Hydrogen Peroxide sterilization processes. Geobacillus Stearothermophilus (ATCC 7953) 10^5 spores per strip.

Instructions for use

1. Pack the envelope in an appropriate package along with material to be sterilized, according to recommended sterilization practices. Place it in those areas you consider a priori more inaccessible to the sterilizing agent. The load center is generally a problematic area.

2. Sterilize as usual.

3. After sterilization process has finished, remove Bionova® BT92 envelope from the package for processing and incubation.

4. Tear up the envelope at one end very carefully under sterility conditions (eg. laminar flow cabinet) and transfer spore strip with a sterile clamp to MC20-2 or MC1020-2 growth medium tube, or any other appropriate growth medium.

IMPORTANT: Use latex gloves and a mask when transferring spore strips from the envelope to the growth medium tube. WARNING! Avoid spore strip contact with any kind of surface including the outer part of growth medium tube and Bionova BT92 envelope.

IMPORTANT: Use a spore strip which has not been subjected to the sterilization process as a positive control each time a processed strip is incubated. Both, processed spore strip and the strip used as positive control must belong to the same batch. Positive control guarantees that the incubation was carried out under appropriate conditions.

5. Incubate processed spore strips along with the strip used as positive control during a maximum of 24 hours between 55-62 °C. It is advisable to make observations every 10 hours.

Color change of MC20-2 or MC1020-2 growth indicating medium to yellow means a failure in the sterilization process. If after 24 hours no color change is visible on MC20-2 or MC1020-2 growth medium, the result is negative (i.e. the sterilization process was effective).

MC20-2 or MC1020-2 growth medium used for incubating the spores strips which have not been sterilized (positive control) should turn to yellow in order for the results to be valid.