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Polyester sutures with spores

SKU: WP90/6 Category: Tag:
Brand

Bionova

Process

Hydrogen Peroxide

Packaging

100

Microorganism

Geobacillus Stearothermophilus (ATCC 7953)

Population

10^6

Conditions

See Product Description

Regulations

IRAM 37102-1, ISO 11138-1

Diameter

0.30 x 50 mm

Description

WP90/6 / Polyester suture with Geobacillus stearothermophilus (ATCC 7953) 10^6 spores for Plasma or Vaporized Hydrogen Peroxide sterilization processes.

Bionova® WP90/6 Polyester Sutures were specifically designed to control Plasma of Vaporized Hydrogen Peroxide (VH2O2) sterilization processes. Polyester sutures should be incubated in Bionova® MC20 or MC1020 growth medium tube, or in an appropriate culture medium that enables Geobacillus stearothermophilus growth.

If the sterilization process was not successful, MC20 or MC1020 culture medium will turn to yellow after incubation at 60±2 °C, thus indicating the presence of living Geobacillus stearothermophilus spores in the suture.

If the sterilization process was correct, MC20 or MC1020 medium will remain its original colour. Final readout should be carried out after a 7-day medium incubation at 60±2 °C.

WARNING: Do not use the polyester sutures to control sterilization processes other than Plasma or Vaporized Hydrogen Peroxide. Do not reuse the sutures

Directions for use

1. In sterility conditions and using a sterile clamp, take the polyester suture out of its hermetic bag. This procedure prevents contamination of the sutures that will not be utilized in this cycle.

2. Place the suture along with the material to be sterilized. Polyester sutures were designed to be used in small lumens, or in areas where a spore strip could not be used because of its dimensions.

3. Sterilize as usual.

4. Once the sterilization process has finished, remove the Bionova® suture from its processing and incubation place.

5. In sterility conditions and using a sterile clamp, transfer the polyester suture to MC20 or MC1020 culture medium tube or to any other appropriate culture medium. Place the suture in a position so that it is completely covered by the culture medium.

WARNING: Use latex gloves and chin strap when transferring the suture to the culture medium tube. Avoid contact of the polyester sutures with any kind of surface including the outer part of culture medium tube.

IMPORTANT: Use a non-sterilized polyester suture as positive control to ensure that incubation conditions were met.

6. Incubate processed sutures along with the positive control for a maximum of 72 hours at 60±2 °C. MC20 or MC1020 culture medium used for incubating the non-sterilized polyester sutures (positive control) should turn to yellow for results to be valid. Record the positives and discard them immediately as indicated below.

WARNING: Do not reuse the sterilizer until polyester sutures growth results are negative (MC20 or MC1020 culture medium containing processed polyester sutures remains its orginal colour).

Description

WP90/6 / Polyester suture with Geobacillus stearothermophilus (ATCC 7953) 10^6 spores for Plasma or Vaporized Hydrogen Peroxide sterilization processes.

Bionova® WP90/6 Polyester Sutures were specifically designed to control Plasma of Vaporized Hydrogen Peroxide (VH2O2) sterilization processes. Polyester sutures should be incubated in Bionova® MC20 or MC1020 growth medium tube, or in an appropriate culture medium that enables Geobacillus stearothermophilus growth.

If the sterilization process was not successful, MC20 or MC1020 culture medium will turn to yellow after incubation at 60±2 °C, thus indicating the presence of living Geobacillus stearothermophilus spores in the suture.

If the sterilization process was correct, MC20 or MC1020 medium will remain its original colour. Final readout should be carried out after a 7-day medium incubation at 60±2 °C.

WARNING: Do not use the polyester sutures to control sterilization processes other than Plasma or Vaporized Hydrogen Peroxide. Do not reuse the sutures

Directions for use

1. In sterility conditions and using a sterile clamp, take the polyester suture out of its hermetic bag. This procedure prevents contamination of the sutures that will not be utilized in this cycle.

2. Place the suture along with the material to be sterilized. Polyester sutures were designed to be used in small lumens, or in areas where a spore strip could not be used because of its dimensions.

3. Sterilize as usual.

4. Once the sterilization process has finished, remove the Bionova® suture from its processing and incubation place.

5. In sterility conditions and using a sterile clamp, transfer the polyester suture to MC20 or MC1020 culture medium tube or to any other appropriate culture medium. Place the suture in a position so that it is completely covered by the culture medium.

WARNING: Use latex gloves and chin strap when transferring the suture to the culture medium tube. Avoid contact of the polyester sutures with any kind of surface including the outer part of culture medium tube.

IMPORTANT: Use a non-sterilized polyester suture as positive control to ensure that incubation conditions were met.

6. Incubate processed sutures along with the positive control for a maximum of 72 hours at 60±2 °C. MC20 or MC1020 culture medium used for incubating the non-sterilized polyester sutures (positive control) should turn to yellow for results to be valid. Record the positives and discard them immediately as indicated below.

WARNING: Do not reuse the sterilizer until polyester sutures growth results are negative (MC20 or MC1020 culture medium containing processed polyester sutures remains its orginal colour).

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