The post Spore ampoule for Dry Heat sterilization processes first appeared on Terragene.

Bionova® BT24 Biological Indicator is specifically designed for monitoring of steam sterilization processes at 110 -121 ºC.

If the sterilization process was not successful, the indicator medium will turn to yellow after incubation between 35-39 ºC, thus indicating the presence of living Bacillus subtilis spores.

If the sterilization process was effective, the indicator medium will remain the original color after incubation. The final readout should be performed after 48 hours of incubation between 35-39 ºC.

Instructions for use

1. Take the Bionova® BT24 Ampoules out of the refrigerator and leave them at room temperature during not longer than ten minutes.

2. Place the Bionova® BT24 Ampoules inside a container with the liquid being sterilized or in a container with liquid similar to that containing the liquid to be sterilized. This container should be of the same size and have the same volume as the one containing the liquid being sterilized.

3. Perform sterilization cycle.

4. Once the sterilization process has finished, open the sterilizer door, wait five minutes and then remove the biological indicator from the container. WARNING: Wear safety glasses and gloves when removing the Bionova® BT24 Biological Indicator from the sterilized container.

5. Incubate the processed biological indicator, a negative control and a non-sterilized biological indicator as a positive control, during 48 hours between 35-39 ºC.

The positive control ensures that the incubation conditions were met.

The negative control, not being exposed to the sterilization process, can be used as a reference color to compare positive and negative results. Conversely, when used with the ampoules during the process, it ensures that any color change produced immediately after sterilization is the result of the process and not due to germination and/or spore growth.

Readout should be performed at convenient intervals of 10 hours.

It is recommended the use of Bionova® IC10/20 Dual Incubator or similar devices that assure an effective temperature control. WARNING: Traditional culture stoves have thermal profiles that differ from the above recommended incubators, thus their use might affect or delay the Bionova® BT21 Biological Indicators result.

A color change to yellow of the proccesed biological indicator medium means a sterilization process failure has occurred.

If after 48 hours there is no color change in the processed indicators, a final negative result is obtained, meaning the sterilization process was successful. The positive control indicator should turn to yellow for the result to be valid and the negative control should remain the same color.

The post Self-contained spore ampoules for Steam (10^6 spores / 0,6ml) first appeared on Terragene.

Bionova® BT23 Self-Contained spore ampoules has been designed for monitoring Steam sterilization processes at temperatures between 121ºC and 135 ºC.

If the sterilization process was not effective, the indicator medium will turn to yellow after incubation between 55-62 ºC, thus indicating the presence of living Geobacillus stearothermophilus spores.

If the sterilization process was effective, the indicator medium will remain the original color after incubation. The final readout should be performed after 48 hours of incubation between 55-62 ºC.

Instructions for use

1. Take the Bionova® BT23 Ampoules out of the refrigerator and leave them stabilize at room temperature up to ten minutes.

2. Place the Bionova® BT23 Ampoules inside a container with the liquid being sterilized or in a container with liquid similar to that containing the liquid to be sterilized. This container should be the same size and have the same volume as the one containing the liquid being sterilized.

3. Perform sterilization cycle.

4. Once the sterilization process has finished, open the sterilizer door, wait five minutes and remove the biological indicator from the container. WARNING: Wear safety glasses and gloves when removing the Bionova® BT23 Biological Indicator from the sterilized container.

5. Incubate the processed biological indicator, a negative control and a non-sterilized biological indicator as a positive control, during 48 hours between 55-62 ºC.

The positive control ensures that the incubation conditions were met.

The negative control, not being exposed to the sterilization process, can be used as a reference color to compare positive and negative results. Conversely, when used with the ampoules during the process, it ensures that any color change produced immediately after sterilization is the result of the process and not due to germination and/or spore growth.

Readout should be performed at convenient intervals of 12 hours.

It is recommended the use of Bionova® IC10/20 Dual Incubator or similar devices that assure an efficient temperature control. WARNING: Traditional culture stoves have thermal profiles that differ from the above recommended incubators, thus their use might affect or delay the Bionova® BT23 Biological Indicators result.

A color change to yellow of the proccesed biological indicator medium means a sterilization process failure has occurred. If after 48 hours there is no color change in the processed indicators, a final negative result is obtained, meaning the sterilization process was effective. The positive control indicator should turn to yellow for the result to be valid and the negative control should remain the same color.

The post Self-contained spore ampoules for Steam first appeared on Terragene.

Bionova® BT22 Self-Contained spore ampoules have been designed for monitoring Steam sterilization processes at temperatures between 121 ºC and 135 ºC.

If the sterilization process was not effective, the indicator medium will turn to yellow after incubation between 55-62 ºC, thus indicating the presence of living Geobacillus stearothermophilus spores.

If the sterilization process was effective, the indicator medium will remain the original color after incubation. The final readout should be performed after 48 hours of incubation between 55-62 ºC.

Instructions for use

1. Take the Bionova® BT22 Ampoules out of the refrigerator and leave them stabilize at room temperature up to ten minutes.

2. Place the Bionova® BT22 Ampoules inside a container with the liquid being sterilized or in a container with liquid similar to that containing the liquid to be sterilized. This container should be the same size and have the same volume as the one containing the liquid being sterilized.

3. Perform sterilization cycle.

4. Once the sterilization process has finished, open the sterilizer door, wait five minutes and remove the biological indicator from the container. WARNING: Wear safety glasses and gloves when removing the Bionova® BT22 Biological Indicator from the sterilized container.

5. Incubate the processed biological indicator, a negative control and a non-sterilized biological indicator as a positive control, during 48 hours between 55-62 ºC.

The positive control ensures that the incubation conditions were met.

The negative control, not being exposed to the sterilization process, can be used as a reference color to compare positive and negative results. Conversely, when used with the ampoules during the process, it ensures that any color change produced immediately after sterilization is the result of the process and not due to germination and/or spore growth.

Readout should be performed at convenient intervals of 12 hours.

It is recommended the use of Bionova® IC10/20 Dual Incubator or similar devices that assure an efficient temperature control. WARNING: Traditional culture stoves have thermal profiles that differ from the above recommended incubators, thus their use might affect or delay the Bionova® BT22 Biological Indicators result.

A color change to yellow of the proccesed biological indicator medium means a sterilization process failure has occurred. If after 48 hours there is no color change in the processed indicators, a final negative result is obtained, meaning the sterilization process was effective.

The positive control indicator should turn to yellow for the result to be valid and the negative control should remain the same color.

The post Self-contained spore ampoules for Steam (10^6 spores / 0,25ml) first appeared on Terragene.